Common cell lines

Human cell lines
National Cancer Institute 60 cancer cell lines
ESTDAB database
DU145 (Prostate cancer)
Lncap (Prostate cancer)
MCF-7 (breast cancer)
MDA-MB-438 (breast cancer)
PC3 (Prostate cancer)
T47D (breast cancer)
THP-1 (acute myeloid leukemia)
U87 (glioblastoma)
SHSY5Y Human neuroblastoma cells, cloned from a myeloma
Saos-2 cells (bone cancer)
Primate cell lines
Vero (African green monkey Chlorocebus kidney epithelial cell line initiated 1962)
Rat tumor cell lines
GH3 (pituitary tumor)
PC12 (pheochromocytoma)
Mouse cell lines
MC3T3 (embryonic calvarial)
Plant cell lines
Tobacco BY-2 cells (kept as cell suspension culture, they are model system of plant cell)
Other species cell lines
zebrafish ZF4 and AB9 cells.
Madin-Darby Canine Kidney (MDCK) epithelia cell line
Xenopus A6 kidney epithelil cells

cell culture Established human cell lines

Cell lines that originate with humans have been somewhat controversial in bioethics, as they may outlive their parent organism and later be used in the discovery of lucrative medical treatments. In the pioneering decision in this area, the Supreme Court of California held in Moore v. Regents of the University of California that human patients have no property rights in cell lines derived from organs removed with their consent.

Generation of hybridomas
For more details on this topic, see Hybridoma.
It is possible to fuse normal cells with an immortalised cell line. This method is used to produce monoclonal antibodies. In brief, lymphocytes isolated from the spleen (or possibly blood) of an immunised animal are combined with an immortal myeloma cell line (B cell lineage) to produce a hybridoma which has the antibody specificity of the primary lymphoctye and the immortality of the myeloma. Selective growth medium (HA or HAT) is used to select against unfused myeloma cells; primary lymphoctyes die quickly in culture and only the fused cells survive. These are screened for production of the required antibody, generally in pools to start with and then after single cloning.
[Applications of cell culture
Mass culture of animal cell lines is fundamental to the manufacture of viral vaccins and many products of biotechnology. Biological products produced by recombinant DNA (rDNA) technology in animal cell cultures include enzymes, synthetic hormones, immunobiologicals(monoclonal antibodies, interleukins, lymphokines), and anticancer agents. Although many simpler proteins can be produced using rDNA in bacterial cultures, more complex proteins that are glycosylated (carbohydrate-modified) currently must be made in animal cells. An important example of such a complex protein is the hormone erythropoietin. The cost of growing mammalian cell cultures is high, so research is underway to produce such complex proteins in insect cells or in higher plants.
Tissue culture and engineering
Cell culture is a fundamental component of tissue culture and tissue engineering, as it establishes the basics of growing and maintaining cells ex vivo.
Vaccines
Vaccines for polio, measles, mumps, rubella, and chickenpox are currently made in cell cultures. Due to the H5N1 pandemic threat, research into using cell culture for influenza vaccines is being funded by the United States government. Novel ideas in the field include recombinant DNA-based vaccines, such as one made using human adenovirus (a common cold virus) as a vector, or the use of adjuvants

Cell culture

Cell culture is the process by which cells are grown under controlled conditions. In practice the term "cell culture" has come to refer to the culturing of cells derived from multicellular eukaryotes, especially animal cells. The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture.
Animal cell culture became a common laboratory technique in the mid-1900s,but the concept of maintaining live cell lines separated from their original tissue source was discovered in the 19th century.

History:
The 19th-century English physiologist Sydney Ringer developed salt solutions containing the chlorides of sodium, potassium, calcium and magnesium suitable for maintaining the beating of an isolated animal heart outside of the body.In 1885 Wilhelm Roux removed a portion of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the principle of tissue culture.Ross Granville Harrison, working at Johns Hopkins Medical School and then at Yale University, published results of his experiments from 1907-1910, establishing the methodology of tissue culture
Cell culture techniques were advanced significantly in the 1940s and 1950s to support research in virology. Growing viruses in cell cultures allowed preparation of purified viruses for the manufacture of vaccines. The Salk polio vaccine was one of the first products mass-produced using cell culture techniques. This vaccine was made possible by the cell culture research of John Franklin Enders, Thomas Huckle Weller, and Frederick Chapman Robbins, who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures.
Concepts in mammalian cell culture:
Isolation of cells
Cells can be isolated from tissues for ex vivo culture in several ways. Cells can be easily purified from blood, however only the white cells are capable of growth in culture. Mononuclear cells can be released from soft tissues by enzymatic digestion with enzymes such as collagenase, trypsn, or pronase, which break down the extracellular matrix. Alternatively, pieces of tissue can be placed in growth media, and the cells that grow out are available for culture. This method is known as explant culture.
Cells that are cultured directly from a subject are known as primary cells. With the exception of some derived from tumors, most primary cell cultures have limited lifespan. After a certain number of population doublings cells undergo the process of senescence and stop dividing, while generally retaining viability.
An established or immortalised cell line has acquired the ability to proliferate indefinitely either through random mutation or deliberate modification, such as artificial of the telomerase gene. There are numerous well established cell lines representative of particular cell types.
Maintaining cells in culture
Cells are grown and maintained at an appropriate temperature and gas mixture (typically, 37°C, 5% CO2 for mammalian cells) in a cell incubator. Culture conditions vary widely for each cell type, and variation of conditions for a particular cell type can result in different phenotypes being expressed.
Aside from temperature and gas mixture, the most commonly varied factor in culture systems is the growth medium. Recipes for growth media can vary in pH, glucose concentration, growth factors, and the presence of other nutrients. The growth factors used to supplement media are often derived from animal blood, such as calf serum. One complication of these blood-derived ingredients is the potential for contamination of the culture with viruses or prions, particularly in biotechnology medical applications. Current practice is to minimize or eliminate the use of these ingredients wherever possible, but this cannot always be accomplished.
Cells can be grown in suspension or adherent cultures. Some cells naturally live in suspension, without being attached to a surface, such as cells that exist in the bloodstream. There are also cell lines that have been modified to be able to survive in suspension cultures so that they can be grown to a higher density than adherent conditions would allow. Adherent cells require a surface, such as tissue culture plastic or microcarrier, which may be coated with extracellular matrix components to increase adhesion properties and provide other signals needed for growth and differentiation. Most cells derived from solid tissues are adherent. Another type of adherent culture is organotypic culture which involves growing cells in a three-dimensional environment as opposed to two-dimensional culture dishes. This 3D culture system is biochemically and physiologically more similar to in vivo tissue, but is technically challenging to maintain because of many factors (e.g. diffusion).
Cell line cross-contamination
Cell line cross-contamination can be a problem for scientists working with cultured cells. Studies suggest that anywhere from 15–20% of the time, cells used in experiments have been misidentified or contaminated with another cell line.Problems with cell line cross contamination have even been detected in lines from the NCI-60 panel, which are used routinely for drug-screening studies. Major cell line repositories including the American Type Culture Collection (ATCC) and the German Collection of Microorganisms and Cell Cultures (DSMZ) have received cell line submissions from researchers that were misidentified by the researcher.Such contamination poses a problem for the quality of research produced using cell culture lines, and the major repositories are now authenticating all cell line submissions.ATCC uses short tandem repeat (STR) DNA fingerprinting to authenticate its cell lines.
To address this problem of cell line cross-contamination, researchers are encouraged to authenticate their cell lines at an early passage to establish the identity of the cell line. Authentication should be repeated before freezing cell line stocks, every two months during active culturing and before any publication of research data generated using the cell lines. There are many methods for identifying cell lines including isoenzyme analysis, human lymphocyte antigen (HLA) typing and STR analysis.

Manipulation of cultured cells
As cells generally continue to divide in culture, they generally grow to fill the available area or volume. This can generate several issues:
Nutrient depletion in the growth media
Accumulation of apoptotic/necrotic (dead) cells.
Cell-to-cell contact can stimulate cell cycle arrest, causing cells to stop dividing known as contact inhibition or senescence.
Cell-to-cell contact can stimulate cellular differentiation.
Among the common manipulations carried out on culture cells are media changes, passaging cells, and transfecting cells. These are generally performed using tissue culture methods that rely on sterile technique. Sterile technique aims to avoid contamination with bacteria, yeast, or other cell lines. Manipulations are typically carried out in a biosafety hood or laminar flow cabinet to exclude contaminating micro-organisms. Antibiotics (e.g. penicillin and streptomycin) and antifungals (e.g. Amphotericin B) can also be added to the growth media.
As cells undergo metabolic processes, acid is produced and the pH decreases. Often, a pH indicator is added to the medium in order to measure nutrient depletion.
Media changes
In the case of adherent cultures, the media can be removed directly by aspiration and replaced.
Passaging cells
Main article: Passaging
Passaging (also known as subculture or splitting cells) involves transferring a small number of cells into a new vessel. Cells can be cultured for a longer time if they are split regularly, as it avoids the senescence associated with prolonged high cell density. Suspension cultures are easily passaged with a small amount of culture containing a few cells diluted in a larger volume of fresh media. For adherent cultures, cells first need to be detached; this is commonly done with a mixture of trypsin-EDTA, however other enzyme mixes are now available for this purpose. A small number of detached cells can then be used to seed a new culture.
Transfection and transduction
Main article: transfection
Main article: transformation (genetics)
Another common method for manipulating cells involves the introduction of foreign DNA by transfection. This is often performed to cause cells to express a protein of interest. More recently, the transfection of RNAi constructs have been realized as a convenient mechanism for suppressing the expression of a particular gene/protein.
DNA can also be inserted into cells using viruses, in methods referred to as transduction, infection or transformation. Viruses, as parasitic agents, are well suited to introducing DNA into cells, as this is a part of their normal course of reproduction.

BioCyc database collection

The BioCyc database collection is a biological database describing genome and pathway information for hundreds of organisms. It is maintained by SRI Internationl, in in Menlo Park, California.
Two databases within BioCyc are highly curated, meaning they have received extensive manual updating with information from the scientific literature. Those databases are EcoCyc and MetaCyc.
The remaining BioCyc databases were generated computationally to predict what metabolic pathways are present in these organisms. In some cases, the databases were refined manually after their generation.
BioCyc databases rely on a software system called Pathway Tools for their initial generation, subsequent updating, and for querying their content. The databases can also be installed locally.
All BioCyc databases share the same database schema, which facilitates comparisons across the databases.

Bio-fermentation technology

1. Advantages of bio-fermentation:
   Standardization:
   Highly controlled by scientific means during its growth and "standardized", every batch is virtually identical and, in a sense, "perfect." This is very important in pharmaceutical terms because without standardization, it is difficult or impossible to develop drug-type standards for substances like Cordyceps. Once a herb can be standardized, all kinds of studies can be conducted that will be accepted by the scientific community. However, standardization, by itself, does not make a product effective. Cost
   Products can be produced more cheaply by bio-fermentation. High quality wild Cordyceps is rare and now sells for well over a thousand dollars a pound in cities like Hong Kong, Tokyo, New York or London. The culture-grown Cordyceps is available at a third of the cost, with approximately the same benefits. Fermentation technology makes this substance more readily available.
   Vegetarian:
   Wild Cordyceps, by weight, is mostly caterpillar. For vegetarians, and those who do not want to consume caterpillars, the new technology provides a solution. The fermentation technology does not include the caterpillar in the growing process. The fungus is grown without the use of animal nutrients and the result is a 100% pure "vegetarian" health product.

Bacterial conjugation Mechanism

The prototype for conjugative plasmids is the F-plasmid, also called the F-factor. The F-plasmid is an episome (a plasmid that can integrate itself into the bacterial chromosome by homologous recombination) of about 100 kb length. It carries its own origin of replication, the oriV, as well as an origin of transfer, or oriT. There can only be one copy of the F-plasmid in a given bacterium, either free or integrated (two immediately before cell division). The host bacterium is called F-positive or F-plus (denoted F+). Strains that lack F plasmids are called F-negative or F-minus (F-).
Among other genetic information, the F-plasmid carries a tra and a trb locus, which together are about 33 kb long and consist of about 40 genes. The tra locus includes the pilin gene and regulatory genes, which together form pili on the cell surface, polymeric proteins that can attach themselves to the surface of F- bacteria and initiate the conjugation. Though there is some debate on the exact mechanism, the pili themselves do not seem to be the structures through which the actual exchange of DNA takes place. This has been shown through tests in which the pilus are allowed to make contact, then denatured by SDS detergent. DNA transformation still proceeds. Several proteins coded for in the tra or trb loci seem to open a channel between the bacteria. It is thought that the traD enzyme located at the base of the pilus is involved in DNA exchange, by iniating membrane fusion. .
When conjugation is initiated, via a mating signal, a relaxase enzyme creates a nick in one plasmid DNA strand at the origin of transfer, or oriT. The relaxase may work alone or in a complex of over a dozen proteins, known collectively as a relaxosome. In the F-plasmid system, the relaxase enzyme is called TraI and the relaxosome consists of TraI, TraY, TraM, and the integrated host factor, IHF. The transferred, or T-strand, is unwound from the duplex plasmid and transferred into the recipient bacterium in a 5'-terminus to 3'-terminus direction. The remaining strand is replicated, either independent of conjugative action (vegetative replication, beginning at the oriV) or in concert with conjugation (conjugative replication similar to the rolling circle replication of lambda phage). Conjugative replication may necessitate a second nick before successful transfer can occur. A recent report claims to have inhibited conjugation with chemicals that mimic an intermediate step of this second nicking event.
If the F-plasmid becomes integrated into the host genome, donor chromosomal DNA may be transferred along with plasmid DNA.The certain amount of chromosomal DNA that is transferred depends on how long the bacteria remain in contact; for common laboratory strains of E. coi the transfer of the entire bacterial chromosome takes about 100 minutes. The transferred DNA can be integrated into the recipient genome via homologous recombination.
A culture of cells containing non-integrated F plasmids usually contains a few that have accidentally become integrated, and these are responsible for those low-frequency chromosomal gene transfers which do occur in such cultures. Some strains of bacteria with an integrated F-plasmid can be isolated and grown in pure culture. Because such strains transfer chromosomal genes very efficiently, they are called Hfr (high frequency of recombination). The E. coli genome was originally mapped by interrupted mating experiments, in which various Hfr cells in the process of conjugation were sheared from recipients after less than 100 minutes (initially using a Waring blender) and investigating which genes were transferred

Bacterial conjugation

'Bacterial conjugation' is the transfer of genetic material between bacteria through direct cell to cell contact, or through a bridge-like connection between the two cells. Discovered in 1946 by Joshua Lederberg and Edward Tatum, conjugation is a mechanism of horizontal gene transfer—as are transformation and transduction—although these mechanisms do not involve cell-to-cell contact.
Bacterial conjugation is often incorrectly regarded as the bacterial equivalent of sexual reproduction or mating. At best, it can be considered to be a limited bacterial version of sex, since it involves some genetic exchange. In order to perform conjugation, one of the bacteria, the donor, must play host to a conjugative or mobilizable genetic element, most often a conjugative or mobilizable plasmid or transposon Most conjugative plasmids have systems ensuring that the recipient cell does not already contain a similar element.
The genetic information transferred is often beneficial to the recipient cell. Benefits may include antibiotic resistance, other xenobiotic tolerance, or the ability to utilize a new metabolite.Such beneficial plasmids may be considered bacterial endosymbionts. Some conjugative elements may also be viewed as genetic parasites on the bacterium, and conjugation as a mechanism that was evolved by the mobile element to spread itself into new hosts.

Biotechnology industrial park

Biotechnology industrial park (BIP, also called Bio-industrial park, Eco-industrial Clusters, bioincubators is a special form of industrial park that specialized in biotechnology.
Function
Such industrial park usually hosted a cluster of companies that they together function as a biorefinery. A biorefinery is a biomass equivalent of an oil refinery, that these companies refines bio materials like soil or wasted oils and produces an array of bioproducts

International Assessment of Agricultural Science and Technology for Development

The International Assessment of Agricultural Knowledge, Science and Technology for Development (IAASTD) is an international effort initiated by the World Bank that evaluated the relevance, quality and effectiveness of agricultural knowledge, science, and technology (AKST), and the effectiveness of public and private sector policies and institutional arrangements.
The project developed out of a consultative process involving 900 participants and 110 countries. The IAASTD was launched as an intergovernmental process, under the co-sponsorship of the FAO, GEF, UNDP, UNEP, UNESCO, the World Bank and WHO.
The IAASTD was a three-year collaborative effort (2005-2007) that assessed AKST with respect to meeting development and sustainability goals of reducing hunger and poverty, improving nutrition, health and rural livelihoods, and facilitating social and environmental sustainability
The results and conclusions of the project were reviewed and ratified during the Intergovernmental Plenary Meeting held 7-12 April, 2008, in Johannesburg, South Africa.

Kelvin probe force microscope

Kelvin probe force microscopy (KPFM), also known as surface potential microscopy, is a noncontact variant of atomic force microscopy (AFM) that was invented in 1991.With KPFM, the work function of surfaces can be observed at atomic or molecular scales. The work function relates to many surface phenomena, including catalytic activity, reconstruction of surfaces, doping and band-bending of semiconductors, charge trapping in dielectrics and corrosion. The map of the work function produced by KPFM gives information about the composition and electronic state of the local structures on the surface of a solid.
KPFM is a scanning probe method where the potentil offset between a probe tip and a surface can be measured using the same principle as a macroscopic Kelvin probe. The cantilever in the AFM is a referen electrode that forms a capacitor with the surface, over which it is scanned laterally at a constant separation. The cantilever is not piezoelectrically driven at its mechanical resonance frequency ω0 as in normal AFM although an alternating current (AC) voltage is applied at this frequency.
When there is a direct-current (DC) potential difference between the tip and the surface, the AC+DC voltage offset will cause the cantilever to vibrate. The origin of the force can be understood by considering that the energy of the capacitor formed by the cantilever and the surface is

plus terms at DC. Only the cross-term proportional to the VDC·VAC product is at the resonance frequency ω0. The resulting vibration of the cantilever is detected using usual scanned-probe microscopy methods (typically involving a diode laser and a four-quadrant detector). A null circuit is used to drive the DC potential of the tip to a value which minimizes the vibration. A map of this nulling DC potential versus the lateral position coordinate therefore produces an image of the work function of the surface.
A related technique, electrostatic force microscopy (EFM), directly measures the force produced on a charged tip by the electric field emanating from the surface. EFM operates much like magneticforcemicroscopy in that the frequency shift or amplitude change of the cantilever oscillation is used to detect the electric field. However, EFM is much more sensitive to topographic artifacts than KFPM and has not proven as useful. Both EFM and KPFM require the use of conductive cantilevers, typically metal-coated silicon or silicon nitride

Bionic architecture

Bionic architecture is a movement for the design and construction of expressive buildings whose layout and lines borrow from natural (i.e. biological) forms. The movement began to mature in the early 21st century, and thus in early designs research was stressed over practicality. Bionic architecture sets itself in opposition to traditional rectangular layouts and design schemes by using curved forms and surfaces reminiscent of structures in biology and fractal mathematics. One of the tasks set themselves by the movement's early pioneers was the development of aesthetic and economic justifications for their approach to architecture.
Related terms:

Bionics
Eco-tech
Organi-tech
Bio-tech
Biourbanism
Architects of Bionic architecture
Greg Lynn
Bates Smart
Nicholas Grimshaw
Santiago Calatrava
Norman Foster
Ken Yeang
Daniel Libeskind
Jan Kaplický

bioeconomics

Bioeconomics is the study of the dynamics of living resources using economic models. It is an attempt to apply the methods of environmental economics and ecological economics to empirical biology.[citation needed] Bioeconomics applies optimal control methods to mathematical models using environmental and ecological elements for resource protection issues relating to resource economics.
Bioeconomics is the science determining the socioeconomic activity threshold for which a biological system can be effectively and efficiently utilised without destroying the conditions for its regeneration and therefore its sustainability

Environmental impacts

Water
Industrialized agriculture with its high yield varieties are extremely water intensive. In the US, agriculture consumes 85% of all fresh water resources. For example, the Southwest uses 36% of the nations water while at the same time only receiving 6% of the country's rainfall.[citation needed] Only 60% of the water used for irrigation comes from surface water supplies. The other 40% comes from underground aquifers that are being used up in a way similar to topsoil that makes the aquifers,[citation needed] as Pfeiffer says, “for all intents and purposes non renewable resources.”[citation needed] The Ogallala Aquifer is essential to a huge portion of central and southwest plain states, but has been at annual overdrafts of 130-160% in excess of replacement. This irrigation source for America's bread basket will become entirely unproductive in another 30 years or so.[citation needed]
Likewise, rivers are drying up at an alarming rate. In 1997, the lower parts of China’s Yellow Rivrwere dry for a record 226 days. Over the past ten years, it has gone dry an average of 70 days a year.[citation needed] Famous lifelines such as the Nile and Ganges along with countless other rivers are sharing in the same fate.[citation needed] The Aral Sea has lost half its area and two-thirds its volume due to river diversion for cotton production.
Also the water quality is being compromised. In the Aral Sea, water salinization has wiped out all native fish, leaving an economy even more dependent on the agricultural model that originated the problem.[citation needed]
Fish are disappearing through another form of agricultural run off as well.[citation needed] When nitrogen-intensive fertilizers wash into waterways it results in an explosion of algae and other microorganisms that lead to oxygen depletion resulting in “dead zones”, killing off fish and other creatures.[citation needed]
Biodiversity
The spread of Green Revolution agriculture affected both agricultural biodiversity and wild biodiversity[citation needed]. There is little disagreement[citation needed] that the Green Revolution acted to reduce agricultural biodiversity, as it relied on just a few high-yield varieties of each crop.
This has led to concerns about the susceptibility of a food supply to pathogens that cannot be controlled by agrochemicals, as well as the permanent loss of many valuable genetic traits bred into traditional varieties over thousands of years. To address these concerns, massive seed banks such as Consultative Group on International Agricultural Research’s (CGIAR) International Plant Genetic Resources Institute (now Bioversity International) have been established (see Svalbard Global Seed Vault).
There are varying opinions about the effect of the Green Revolution on wild biodiversity. One hypothesis speculates that by increasing production per unit of land area, agriculture will not need to expand into new, uncultivated areas to feed a growing human population[citation needed]. A counter-hypothesis speculates that biodiversity was sacrificed because traditional systems of agriculture that were displaced sometimes incorporated practices to preserve wild biodiversity, and because the Green Revolution expanded agricultural development into new areas where it was once unprofitable or too arid.[citation needed]
Nevertheless, the world community has clearly acknowledged the negative aspects of agricultural expansion as the 1992 Rio Treaty, signed by 189 nations, has generated numerous national Biodiversity Action Plans which assign significant biodiversity loss to agriculture's expansion into new domains

Agricultural production and food security

Technologies
The projects within the Green Revolution spread technologies that had already existed, but had not been widely used outside industrialized nations. These technologies included pesticides, irrigation projects, synthetic nitrogen fertilizer and improved crop varieties developed through the conventional, science-based methods available at the time.
The novel technological development of the Green Revolution was the production of novel wheat cultivars. Agronomists bred cultivars of maize, whet, and rice that are generally referred to as HYVs or “high-yielding varieties”. HYVs have higher nitrogen-absorbing potential than other varieties. Since cereals that absorbed extra nitrogen would typically lodge, or fall over before harvest, semi-dwarfing genes were bred into their genomes. A Japanese dwarf wheat cultivar (Norin 10 wheat), which was sent to Washingto, D.C. by Cecil Salmon, was instrumental in developing Green Revolution wheat cultivars. IR8, the first widely implemented HYV rice to be developed by IRRI, was created through a cross between an Indonesian variety named “Peta” and a Chinese variety named “Dee-geo-woo-gen.”
With advances in molecular genetics, the mutant gens responsible for Arabidopsis genes (GA 20-oxidase, ga1, ga1-3, wheat reduced-height genes (Rht) and a rice semidwarf gene (sd1)were cloned. These were identified as gibberellin biosynthesis genes or cellular signaling component genes. Stem growth in the mutant background is significantly reduced leading to the dwarf phenotype. Photosynthetic investment in the stem is reduced dramatically as the shorter plants are inherently more stable mechanically. Assimilates become redirected to grain production, amplifying in particular the effect of chemical fertilizers on commercial yield.
HYVs significantly outperform traditional varieties in the presence of adequate irrigation, pesticides, and fertilizers. In the absence of these inputs, traditional varieties may outperform HYVs.

Green Revolution

Green Revolution refers to the transformation of agriculture that began in 1945. One significant factor in this revolution was the Mexican government's request to establish an agricultural research station to develop more varieties of wheat that could be used to feed the rapidly growing population of the country.
In 1943, Mexico imported half its wheat, but by 1956, the Green Revolution had made Mexico self-sufficient; by 1964, Mexico exported half a million tons of wheat. The associated transformation has continued as the result of programs of agricultural research, extension, and infrastructural development. These programs were instigated and largely funded by the Rockefeller Foundation, along with the Ford Foundation and among other major agencies.
The Green Revolution allowed food production to keep pace with worldwide population growth. The Green Revolution has had major social and ecological impacts, making it a popular topic of study among sociologists[citation needed]
The term "Green Revolution" was first used in 1968 by former USAID director William Gaud, who noted the spread of the new technologies and said,
"These and other developments in the field of agriculture contain the makings of a new revolution. It is not a violent Red Revolution like that of the Soviets, nor is it a White Revolution like that of the Shah of Iran. I call it the Green Revolution

Biological engineering

Biotechnology is being used to engineer and adapt organisms especially microorganisms in an effort to find sustainable ways to clean up contaminated environments. The elimination of a wide range of pollutants and wastes from the environment is an absolute requirement to promote a sustainable development of our society with low environmental impact. Biological processes play a major role in the removal of contaminants and biotechnology is taking advantage of the astonishing catabolic versatility of microorganisms to degrade/convert such compounds. New methodological breakthroughs in sequencing, genomics, proteomics, bioinformatics and imaging are producing vast amounts of information. In the field of Environmental Microbiology, genome-based global studies open a new era providing unprecedented in silico views of metabolic and regulatory networks, as well as clues to the evolution of degradation pathways and to the molecular adaptation strategies to changing environmental conditions. Functional genomic and metagenomic approaches are increasing our understanding of the relative importance of different pathways and regulatory networks to carbon flu in particular environments and for particular compounds and they will certainly accelerate the development of bioremediation technologies and biotransformation processes.
Marine environments are especially vulnerable since oil spills of coastal regions and the open sea are poorly containable and mitigation is difficult. In addition to pollution through human activities, millions of tons of petroleum enter the marine environment every year from natural seepages. Despite its toxicity, a considerable fraction of petroleum oil entering marine systems is eliminated by the hydrocarbon-degrading activities of microbial communities, in particular by a remarkable recently discovered group of specialists, the so-called hydrocarbonoclastic bacteria (HCCB).

Human Genome

The Human Genome Project is an initiative of the U.S. Department of Energy (“DOE”) that aims to generate a high-quality reference sequence for the entire human genome and identify all the human genes.
The DOE and its predecessor agencies were assigned by the U.S. Congress to develop new energy resources and technologies and to pursue a deeper understanding of potential health and environmental risks posed by their production and use. In 1986, the DOE announced its Human Genome Initiative. Shortly thereafter, the DOE and National Institutes of Health developed a plan for a joint Human Genome Project (“HGP”), which officially began in 1990.
The HGP was originally planned to last 15 years. However, rapid technological advances and worldwide participation accelerated the completion date to 2003 (making it a 13 year project). Already it has enabled gene hunters to pinpoint genes associated with more than 30 disorders.[
Cloning:
Main article: Cloning
Cloning involves the removal of the nucleus from one cell and its placement in an unfertilized egg cell whose nucleus has either been deactivated or removed.
There are two types of cloning:
Reproductive cloning. After a few divisions, the egg cell is placed into a uterus where it is allowed to develop into a fetus that is genetically identical to the donor of the original nucleus.
Therapeutic cloning.The egg is placed into a Petri dish where it develops into embryonic stem cells, which have shown potentials for treating several ailments.
In February 1997, cloning became the focus of media attention when Ian Wilmut and his colleagues at the Roslin Institute announced the successful cloning of a sheep, named Dolly, from the mammary glands of an adult female. The cloning of Dolly made it apparent to many that the techniques used to produce her could someday be used to clone human beings.This stirred a lot of controversy because of its ethical implications.

Medicine

In medicine, modern biotechnology finds promising applications in such areas as

drug production;
pharmacogenomics;
gene therapy; and
genetic testing; Pharmacogenomics
DNA Microarray chip – Some can do as many as a million blood tests at onceMain article: Pharmacogenomics
Pharmacogenomics is the study of how the genetic inheritance of an individual affects his/her body’s response to drugs. It is a coined word derived from the words “pharmacology” and “genomics”. It is hence the study of the relationship between pharmaceuticals and genetics. The vision of pharmacogenomics is to be able to design and produce drugs that are adapted to each person’s genetic makeup.

Pharmacogenomics results in the following benefits:
Development of tailor-made medicines. Using pharmacogenomics, pharmaceutical companies can create drugs based on the proteins, enzymes and RNA molecules that are associated with specific genes and diseases. These tailor-made drugs promise not only to maximize therapeutic effects but also to decrease damage to nearby healthy cells.
More accurate methods of determining appropriate drug dosages. Knowing a patient’s genetics will enable doctors to determine how well his/ her body can process and metabolize a medicine. This will maximize the value of the medicine and decrease the likelihood of overdose.
Improvements in the drug discovery and approval process. The discovery of potential therapies will be made easier using genome targets. Genes have been associated with numerous diseases and disorders. With modern biotechnology, these genes can be used as targets for the development of effective new therapies, which could significantly shorten the drug discovery process.
Better vaccines. Safer vaccines can be designed and produced by organisms transformed by means of genetic engineering. These vaccines will elicit the immune response without the attendant risks of infection. They will be inexpensive, stable, easy to store, and capable of being engineered to carry several strains of pathogen at once.
Pharmaceutical products:

Computer-generated image of insulin hexamers highlighting the threefold symmetry, the zinc ions holding it together, and the histidine residues involved in zinc binding.Most traditional pharmaceutical drugs are relatively simple molecules that have been found primarily through trial and error to treat the symptoms of a disease or illness.[citation needed] Biopharmaceuticals are large biological molecules known as proteins and these usually target the underlying mechanisms and pathways of a malady (but not always, as is the case with using insulin to treat type 1 diabetes mellitus, as that treatment merely addresses the symptoms of the disease, not the underlying cause which is autoimmunity); it is a relatively young industry. They can deal with targets in humans that may not be accessible with traditional medicines. A patient typically is dosed with a small molecule via a tablet while a large molecule is typically injected.

Small molecules are manufactured by chemistry but larger molecules are created by living cells such as those found in the human body: for example, bacteria cells, yeast cells, animal or plant cells.

Modern biotechnology is often associated with the use of genetically altered microorganisms such as E. coli or yeast for the production of substances like synthetic insulin or antibiotics. It can also refer to transgenic animals or transgenic plants, such as Bt corn. Genetically altered mammalian cells, such as Chinese Hamster Ovary (CHO) cells, are also used to manufacture certain pharmaceuticals. Another promising new biotechnology application is the development of plant-made pharmaceuticals.

Biotechnology is also commonly associated with landmark breakthroughs in new medical therapies to treat hepatitis B, hepatitis C, cancers, arthritis, haemophilia, bone fractures, multiple sclerosis, and cardiovascular disorders. The biotechnology industry has also been instrumental in developing molecular diagnostic devices that can be used to define the target patient population for a given biopharmaceutical. Herceptin, for example, was the first drug approved for use with a matching diagnostic test and is used to treat breast cancer in women whose cancer cells express the protein HER2.

Modern biotechnology can be used to manufacture existing medicines relatively easily and cheaply. The first genetically engineered products were medicines designed to treat human diseases. To cite one example, in 1978 Genentech developed synthetic humanized insulin by joining its gene with a plasmid vector inserted into the bacterium Escherichia coli. Insulin, widely used for the treatment of diabetes, was previously extracted from the pancreas of abattoir animals (cattle and/or pigs). The resulting genetically engineered bacterium enabled the production of vast quantities of synthetic human insulin at relatively low cost[8]. According to a 2003 study undertaken by the International Diabetes Federation (IDF) on the access to and availability of insulin in its member countries, synthetic 'human' insulin is considerably more expensive in most countries where both synthetic 'human' and animal insulin are commercially available: e.g. within European countries the average price of synthetic 'human' insulin was twice as high as the price of pork insulin[9]. Yet in its position statement, the IDF writes that "there is no overwhelming evidence to prefer one species of insulin over another" and "[modern, highly-purified] animal insulins remain a perfectly acceptable alternative.

Modern biotechnology has evolved, making it possible to produce more easily and relatively cheaply human growth hormone, clotting factors for hemophiliacs, fertility drugs, erythropoietin and other drugs.[11] Most drugs today are based on about 500 molecular targets. Genomic knowledge of the genes involved in diseases, disease pathways, and drug-response sites are expected to lead to the discovery of thousands more new targets.

Applications of biotech

Biotechnology has applications in four major industrial areas, including health care (medical), crop production and agriculture, non food (industrial) uses of crops and other products (e.g. biodegradable plastics, vegetable oil, biofuels), and environmental uses.

For example, one application of biotechnology is the directed use of organisms for the manufacture of organic products (examples include beer and milk products). Another example is using naturally present bacteria by the mining industry in bioleaching. Biotechnology is also used to recycle, treat waste, clean up sites contaminated by industrial activities (bioremediation), and also to produce biological weapons.

A series of derived terms have been coined to identify several branches of biotechnology, for example:

Bioinformatics is an interdisciplinary field which addresses biological problems using computational techniques, and makes the rapid organization and analysis of biological data possible. The field may also be referred to as computational biology, and can be defined as, "conceptualizing biology in terms of molecules and then applying informatics techniques to understand and organize the information associated with these molecules, on a large scale."[6] Bioinformatics plays a key role in various areas, such as functional genomics, structural genomics, and proteomics, and forms a key component in the biotechnology and pharmaceutical sector.
Blue biotechnology is a term that has been used to describe the marine and aquatic applications of biotechnology, but its use is relatively rare.
Green biotechnology is biotechnology applied to agricultural processes. An example would be the selection and domestication of plants via micropropagation. Another example is the designing of transgenic plants to grow under specific environments in the presence (or absence) of chemicals. One hope is that green biotechnology might produce more environmentally friendly solutions than traditional industrial agriculture. An example of this is the engineering of a plant to express a pesticide, thereby ending the need of external application of pesticides. An example of this would be Bt corn. Whether or not green biotechnology products such as this are ultimately more environmentally friendly is a topic of considerable debate.
Red biotechnology is applied to medical processes. Some examples are the designing of organisms to produce antibiotics, and the engineering of genetic cures through genetic manipulation.
White biotechnology, also known as industrial biotechnology, is biotechnology applied to industrial processes. An example is the designing of an organism to produce a useful chemical. Another example is the using of enzymes as industrial catalysts to either produce valuable chemicals or destroy hazardous/polluting chemicals. White biotechnology tends to consume less in resources than traditional processes used to produce industrial goods.[citation needed] The investments and economic output of all of these types of applied biotechnologies form what has been described as the bioeconomy.

History

Although not normally thought of as biotechnology, agriculture clearly fits the broad definition of "using a biological system to make products" such that the cultivation of plants may be viewed as the earliest biotechnological enterprise. Agriculture has been theorized to have become the dominant way of producing food since the Neolithic Revolution. The processes and methods of agriculture have been refined by other mechanical and biological sciences since its inception. Through early biotechnology, farmers were able to select the best suited and highest-yield crops to produce enough food to support a growing population. Other uses of biotechnology were required as crops and fields became increasingly large and difficult to maintain. Specific organisms and organism by-products were used to fertilize, restore nitrogen, and control pests. Throughout the use of agriculture, farmers have inadvertently altered the genetics of their crops through introducing them to new environments and breeding them with other plants—one of the first forms of biotechnology. Cultures such as those in Mesopotamia, Egypt, and India developed the process of brewing beer. It is still done by the same basic method of using malted grains (containing enzymes) to convert starch from grains into sugar and then adding specific yeasts to produce beer. In this process the carbohydrates in the grains were broken down into alcohols such as ethanol. Ancient Indians also used the juices of the plant Ephedra vulgaris and used to call it Soma.[citation needed] Later other cultures produced the process of Lactic acid fermentation which allowed the fermentation and preservation of other forms of food. Fermentation was also used in this time period to produce leavened bread. Although the process of fermentation was not fully understood until Manish keswani’s work in 1857, it is still the first use of biotechnology to convert a food source into another form.

what is biotechnology

biotech is technology based on biology, agriculture, food science, and medicine. Modern use of the term usually refers to genetic engineering as well as cell- and tissue culture technologies. However, the concept encompasses a wider range and history of procedures for modifying living organisms according to human purposes, going back to domestication of animals, cultivation of plants and "improvements" to these through breeding programs that employ artificial selection and hybridization. By comparison to biotechnology, bioengineering is generally thought of as a related field with its emphasis more on mechanical and higher systems approaches to interfacing with and exploiting living things. United Nations Convention on Biological Diversity defines biotechnology as

"Any technological application that uses biological systems, living organisms, or derivatives thereof, to make or modify products or processes for specific use."
Biotechnology draws on the pure biological sciences (genetics, microbiology, animal cell culture, molecular biology, biochemistry, embryology, cell biology) and in many instances is also dependent on knowledge and methods from outside the sphere of biology (chemical engineering, bioprocess engineering, information technology, biorobotics). Conversely, modern biological sciences (including even concepts such as molecular ecology) are intimately entwined and dependent on